Comparative Genomics of Burkholderia singularis sp. nov., a Low G+C Content, Free-Living Bacterium That Defies Taxonomic Dissection of the Genus Burkholderia

• Published in: Frontiers in microbiology Vol. 8; p. 1679
• Main Authors: Vandamme, Peter, Peeters, Charlotte, De Smet, Birgit, Price, Erin P, Sarovich, Derek S, Henry, Deborah A, Hird, Trevor J, Zlosnik, James E A, Mayo, Mark, Warner, Jeffrey, Baker, Anthony, Currie, Bart J, Carlier, Aurélien
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media 06.09.2017; Frontiers Media S.A
• Subjects: Bacteriology; Biochemistry, Molecular Biology; Burkholderia cepacia complex; Burkholderia pseudomallei complex; Burkholderia singularis; comparative genomics; cystic fibrosis microbiology; Genomics; Life Sciences; Microbiology; Microbiology and Parasitology; whole genome sequence; whole genome sequence; Burkholderia singularis; Burkholderia cepacia complex; comparative genomics; Burkholderia pseudomallei complex; cystic fibrosis microbiology
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2017.01679

Four -like isolates of human clinical origin were examined by a polyphasic taxonomic approach that included comparative whole genome analyses. The results demonstrated that these isolates represent a rare and unusual, novel species for which we propose the name The type strain is LMG 28154 (=CCUG 65685 ). Its genome sequence has an average mol% G+C content of 64.34%, which is considerably lower than that of other species. The reduced G+C content of strain LMG 28154 was characterized by a genome wide AT bias that was not due to reduced GC-biased gene conversion or reductive genome evolution, but might have been caused by an altered DNA base excision repair pathway. can be differentiated from other species by multilocus sequence analysis, MALDI-TOF mass spectrometry and a distinctive biochemical profile that includes the absence of nitrate reduction, a mucoid appearance on Columbia sheep blood agar, and a slowly positive oxidase reaction. Comparisons with publicly available whole genome sequences demonstrated that strain TSV85, an Australian water isolate, also represents the same species and therefore, to date, has been recovered from human or environmental samples on three continents.