Controlled nano-agglomerates as stabile SERS reporters for unequivocal labelling

Biosensors, especially those with a SERS readout, are required for an early and precise healthcare diagnosis. Unreproducible SERS platforms hamper clinical SERS. Here we report a synthetic procedure to obtain stabile, reproducible and robust highly-SERS performing nanocomposites for labelling. We co...

Full description

Saved in:
Bibliographic Details
Published inScientific reports Vol. 12; no. 1; p. 8977
Main Authors Xiao, Can, Mir-Simón, Bernat, Rivera-Gil, Pilar
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 28.05.2022
Nature Publishing Group UK
Nature Portfolio
Subjects
Biosensors
COVID-19
Gold - chemistry
Humans
Labeling
Metal Nanoparticles - chemistry
Nanocomposites
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Spectrum Analysis, Raman - methods
Online AccessGet full text

Cover

More Information
Summary:Biosensors, especially those with a SERS readout, are required for an early and precise healthcare diagnosis. Unreproducible SERS platforms hamper clinical SERS. Here we report a synthetic procedure to obtain stabile, reproducible and robust highly-SERS performing nanocomposites for labelling. We controlled the NPs agglomeration and codification which resulted in an increased number of hot spots, thus exhibiting reproducible and superior Raman enhancement. We studied fundamental aspects affecting the plasmonic thiol bond resulting in pH exhibiting a determining role. We validated their biosensing performance by designing a SERS-based detection assay model for SARS-CoV-2. The limit of detection of our assay detecting the spike RBD was below 10 ng/mL.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-12989-6