Primary muscle cells cultivated in medium conditioned by spinal cord cells show changes in messenger RNA as detected by translation in ovo accompanied by synthesis of extracellular matrix components

Primary muscle cell cultures were prepared from rat embryos and maintained in normal (unconditioned) and spinal cord (conditioned) media. In order to relate translational regulation to the morphological changes associated with each culture condition, mRNA was isolated from both culture variants and...

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Bibliographic Details
Published inBiology of the cell Vol. 59; no. 1; p. 55
Main Authors Schmid, D W, de la Porte, S, Koenig, J
Format Journal Article
LanguageEnglish
Published England 1987
Subjects
Animals
Cells, Cultured
Embryo, Mammalian
Embryo, Nonmammalian
Female
Muscles - cytology
Muscles - metabolism
Oocytes - metabolism
Protein Biosynthesis
Rats
RNA, Messenger - genetics
RNA, Messenger - metabolism
Spinal Cord - physiology
Xenopus
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Summary:Primary muscle cell cultures were prepared from rat embryos and maintained in normal (unconditioned) and spinal cord (conditioned) media. In order to relate translational regulation to the morphological changes associated with each culture condition, mRNA was isolated from both culture variants and subjected to in ovo translation. Xenopus oocytes were injected with mRNA and their incubation media checked for the presence of newly formed proteins coded by the mRNAs and secreted into the medium. Electrophoretograms indicated mRNA-dependent synthesis of several proteins in the molecular mass range of 30-260 kD. To further characterize these proteins, antisera directed against several extracellular matrix proteins were used for immunoprecipitation: antigens recognized by anti-heparan-sulfate-proteoglycan and anti-fibronectin were enhanced in conditioned cells, whereas laminin was found to be reduced.
ISSN:0248-4900
1768-322X
DOI:10.1111/j.1768-322X.1987.tb00515.x