Leaf anatomical changes in Populus trichocarpa, Quercus rubra, Pseudotsuga menziesii and Pinus ponderosa exposed to enhanced ultraviolet-B radiation

• Published in: Physiologia plantarum Vol. 104; no. 3; pp. 385 - 396
• Main Authors: Nagel, L.M, Bassman, J.H. (Washington State Univ., Pullman (USA)), Edwards, G.E, Robberecht, R, Franceshi, V.R
• Format: Journal Article
• Language: English
• Published: Copenhagen Munksgaard International Publishers 01.11.1998; Blackwell
• Subjects: AGOTAMIENTO DEL OZONO; Agricultural and forest climatology and meteorology. Irrigation. Drainage; Agricultural and forest meteorology; Agronomy. Soil science and plant productions; ANATOMIA DE LA PLANTA; ANATOMIE VEGETALE; Biological and medical sciences; BIOMASA; BIOMASS; BIOMASSE; Climatic adaptation. Acclimatization; CONIFERALE; CONIFERALES; FEUILLE; FOTOPERIODISMO; Fundamental and applied biological sciences. Psychology; GENE; General agronomy. Plant production; GENES; HOJAS; LEAF AREA; LEAVES; OZONE DEPLETION; PHOTOPERIODICITE; PHOTOPERIODICITY; Physical agents; PINUS PONDEROSA; PLANT ANATOMY; Plant physiology and development; PLANT TISSUES; PLANTULAS; PLANTULE; POPULUS TRICHOCARPA; PSEUDOTSUGA MENZIESII; QUERCUS RUBRA; RADIACION ULTRAVIOLETA; RAREFACTION DE L'OZONE; RAYONNEMENT ULTRAVIOLET; SEEDLINGS; SUPERFICIE FOLIAR; SURFACE FOLIAIRE; TEJIDOS VEGETALES; TISSU VEGETAL; ULTRAVIOLET RADIATION; Vegetative apparatus, growth and morphogenesis. Senescence; UVB radiation; Interspecific comparison; Quercus rubra; Plant leaf; Populus trichocarpa; Fagaceae; Anatomy; Long term; Pseudotsuga menziesii; Salicaceae; Pinus ponderosa; Dicotyledones; Morphology; Angiospermae; Softwood forest tree; Gymnospermae; Development; Hardwood forest tree; Coniferales; Spermatophyta; Damage; Adaptation
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1034/j.1399-3054.1998.1040314.x

Leaf anatomical characteristics are important in determining the degree of injury sustained when plants are exposed to natural and enhanced levels of ultraviolet‐B (UV‐B) radiation (280–320 nm). The degree to which leaf anatomy can adapt to the increasing levels of UV‐B radiation reaching the earth's surface is poorly understood in most tree species. We examined four tree species, representing a wide range of leaf anatomical characteristics, to determine responses of leaf area, specific leaf weight, and leaf tissue parameters after exposure to ambient and enhanced levels of UV‐B radiation. Seedlings were grown in a greenhouse with photosynthetically active radiation of 39 mol m−2 day−1 and under one of three daily irradiances of biologically effective UV‐B radiation (UV‐BBE) supplied for 10 h per day: (1) approximate ambient level received at Pullman, Washington on June 21 (1 x ); two times ambient (2 x ), or three times ambient (3 x ). We hypothesized the response of each species to UV‐B radiation would be related to inherent anatomical differences. We found that the conifers responded anatomically to nearly an equal degree as the broad‐leaved trees, but that different tissues were involved. Populus trichocarpa, an indeterminate broadleaf species, showed significantly thicker palisade parenchyma in recently mature leaves at the 3 x level and in older leaves under the 2 x level. In addition, individual leaf area was generally greater with increased UV‐B irradiance. Quercus rubra, a semi‐determinate broadleaf species, exhibited significantly thicker palisade parenchyma at the 2 x and 3 x levels as compared to controls. Psuedotsuga menziesii, an evergreen coniferous species with bifacially flattened needles, and Pinus ponderosa, an evergreen coniferous species with a complete hypodermis, showed no significant change in leaf area or specific leaf weight under enhanced UV‐B radiation. Epidermal thickness was unchanged in P. menziesii. However, P. ponderosa increased the thickness and number of hypodermal layers produced, presumably decreasing penetration of UV‐B radiation into the leaf. We concluded that differences in inherent leaf anatomy of the four species examined are important in the responses to enhanced levels of UV‐B radiation.