Separation and determination of epinephrine and dopamine in traditional Chinese medicines by micellar electrokinetic capillary chromatography with laser induced fluorescence detection
A micellar electrokinetic capillary chromatography method with laser‐induced fluorescence detection was developed for the analysis of epinephrine and dopamine after derivatization with 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1,3‐diazole. The optimum derivatization conditions were: 30 mM sodium borate (pH adjust...
Saved in:
Published in | Journal of separation science Vol. 29; no. 13; pp. 2049 - 2055 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.08.2006
WILEY‐VCH Verlag Wiley |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | A micellar electrokinetic capillary chromatography method with laser‐induced fluorescence detection was developed for the analysis of epinephrine and dopamine after derivatization with 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1,3‐diazole. The optimum derivatization conditions were: 30 mM sodium borate (pH adjusted to 8.0 with 1.0 M HCl), reaction time 30 min at 60°C. Baseline separation was achieved within 14 min with a running buffer composed of 10 mM sodium borate + 25 mM sodium dodecyl sulfate (pH adjusted to 9.5 with 0.1 M NaOH) and an applied voltage of 15 kV. Good linearity relationships (correlation coefficients: 0.9991 for epinephrine and 0.9985 for dopamine) between peak areas and concentrations of the analytes were obtained. The detection limits and quantification limits for epinephrine and dopamine were 0.0038 mg/L and 0.013 mg/L, and 0.065 mg/L and 0.020 mg/L, respectively. The method was applied to the analysis of the two compounds in two Chinese medicines with recoveries in the range of 92.6–108.7%. |
---|---|
Bibliography: | ArticleID:JSSC200600073 istex:490F7A3D02319008373922D26EB6BF31F136064C ark:/67375/WNG-TZBSBVVS-7 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1615-9306 1615-9314 |
DOI: | 10.1002/jssc.200600073 |