Separation and determination of epinephrine and dopamine in traditional Chinese medicines by micellar electrokinetic capillary chromatography with laser induced fluorescence detection

• Published in: Journal of separation science Vol. 29; no. 13; pp. 2049 - 2055
• Main Authors: Dong, Yuming, Chen, Hongli, Chen, Yonglei, Hui, Yang, Chen, Xingguo, Hu, Zhide
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.08.2006; WILEY‐VCH Verlag; Wiley
• Subjects: Amphibian Venoms - chemistry; Animals; Biological and medical sciences; Bufanolides - chemistry; Buffers; Chromatography, Micellar Electrokinetic Capillary - methods; Chromatography, Micellar Electrokinetic Capillary - statistics & numerical data; Dioscorea - chemistry; Dopamine; Dopamine - analysis; Dopamine - isolation & purification; Drugs, Chinese Herbal - chemistry; Epinephrine; Epinephrine - analysis; Epinephrine - isolation & purification; Fluorescence; General pharmacology; Hydrogen-Ion Concentration; Laser induced fluorescence detection; Lasers; Medical sciences; Micellar electrokinetic capillary chromatography; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Asia; China; Epinephrine; Fluorescence detector; Chemical analysis; Dopamine; Pharmacognosy; Micellar electrokinetic capillary chromatography; Laser induced fluorescence; Catecholamine; Derivatization; Operating conditions; Folk medicine; Fluorescent labelling; Biogenic amine; Quality control; nephrine / Laser induced fluorescence detection / Micellar electrokinetic capillary chromatography; Quantitative analysis
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200600073

A micellar electrokinetic capillary chromatography method with laser‐induced fluorescence detection was developed for the analysis of epinephrine and dopamine after derivatization with 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1,3‐diazole. The optimum derivatization conditions were: 30 mM sodium borate (pH adjusted to 8.0 with 1.0 M HCl), reaction time 30 min at 60°C. Baseline separation was achieved within 14 min with a running buffer composed of 10 mM sodium borate + 25 mM sodium dodecyl sulfate (pH adjusted to 9.5 with 0.1 M NaOH) and an applied voltage of 15 kV. Good linearity relationships (correlation coefficients: 0.9991 for epinephrine and 0.9985 for dopamine) between peak areas and concentrations of the analytes were obtained. The detection limits and quantification limits for epinephrine and dopamine were 0.0038 mg/L and 0.013 mg/L, and 0.065 mg/L and 0.020 mg/L, respectively. The method was applied to the analysis of the two compounds in two Chinese medicines with recoveries in the range of 92.6–108.7%.