The costa of trichomonads: A complex macromolecular cytoskeleton structure made of uncommon proteins

• Published in: Biology of the cell Vol. 109; no. 6; pp. 238 - 253
• Main Authors: Andrade Rosa, Ivone, Caruso, Marjolly Brigido, Oliveira Santos, Eidy, Gonzaga, Luiz, Zingali, Russolina Benedeta, Vasconcelos, Ana Tereza R, Souza, Wanderley, Benchimol, Marlene
• Format: Journal Article
• Language: English
• Published: England 01.06.2017
• Subjects: Cell Fractionation; Coiled‐coils; Cytoskeleton; Cytoskeleton - chemistry; Cytoskeleton - metabolism; Cytoskeleton - ultrastructure; Mass spectrometry; Microscopy, Electron; Protozoan Proteins - chemistry; Protozoan Proteins - metabolism; Protozoan Proteins - ultrastructure; Tandem Mass Spectrometry; Trichomonadida - chemistry; Trichomonadida - metabolism; Trichomonadida - ultrastructure; Trichomonas vaginalis; Tritrichomonas foetus; Trichomonas vaginalis; Coiled-coils; Cytoskeleton; Mass spectrometry; Tritrichomonas foetus
• ISSN: 0248-4900; 1768-322X
• DOI: 10.1111/boc.201600050

Background Information The costa is a prominent striated fibre that is found in protozoa of the Trichomonadidae family that present an undulating membrane. It is composed primarily of proteins that have not yet been explored. In this study, we used cell fractionation to obtain a highly enriched costa fraction whose structure and composition was further analysed by electron microscopy and mass spectrometry. Results Electron microscopy of negatively stained samples revealed that the costa, which is a periodic structure with alternating electron‐dense and electron‐lucent bands, displays three distinct regions, named the head, neck and body. Fourier transform analysis showed that the electron‐lucent bands present sub‐bands with a regular pattern. An analysis of the costa fraction via one‐ and two‐dimensional electrophoresis and liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) allowed the identification of 54 hypothetical proteins. Fourteen of those proteins were considered to be major components of the fraction. Conclusions The costa of T. foetus is a complex and organised cytoskeleton structure made of a large number of proteins which is assembled into filamentous structures. Some of these proteins exhibit uncharacterised domains and no function related according to gene ontology, suggesting that the costa structure may be formed by a new class of proteins that differ from those previously described in other organisms. Seven of these proteins contain prefoldin domains displaying coiled‐coil regions. This propriety is shared with proteins of the striated fibres of other protozoan as well as in intermediate filaments. Significance Our observations suggest the presence of a new class of the cytoskeleton filaments in T. foetus. We believe that our data could auxiliate in determining the specific locations of these proteins in the distinct regions that compose the costa, as well as to define the functional roles of each component. Therefore, our study will help in the better understanding of the organisation and function of this structure in unicellular organisms. Research article: Distribution of the functional categories of the T. foetus proteins identified by proteomic approaches. (A) Scheme of the methodology used to identify proteins of the costa in an enriched fraction and (B) functional classification of the costa enriched fraction proteins of the T. foetus according to GO showing a high number of the hypothetical proteins which could indicated that the costa could be composed by uncommon proteins.