L929 CELL CONDITIONED MEDIUM PROTECTS RAW264.7 CELLS FROM OXIDATIVE INJURY THROUGH INDUCING ANTIOXIDANT ENZYMES

• Published in: Cytokine (Philadelphia, Pa.) Vol. 12; no. 7; pp. 944 - 950
• Main Authors: Pang, Zhan-Jun, Chen, Yuan, Zhou, Mei
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2000
• Subjects: Animals; Antioxidants; Cell Line; Cell Survival - drug effects; Culture Media, Conditioned; Enzyme Induction; Gene Expression - drug effects; Glutathione Peroxidase - biosynthesis; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Macrophage Colony-Stimulating Factor - physiology; macrophage colony-stimulating factor/oxidative stress/antioxidant enzyme/RAW264.7 cell line/L929 cell line; Macrophages - cytology; Macrophages - drug effects; Mice; Oxidants - pharmacology; Oxidative Stress; RNA, Messenger; Superoxide Dismutase - biosynthesis; Superoxide Dismutase - metabolism; tert-Butylhydroperoxide - pharmacology; macrophage colony-stimulating factor/oxidative stress/antioxidant enzyme/RAW264.7 cell line/L929 cell line
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1006/cyto.2000.0683

We have previously found that L929 cell conditioned medium (L929-CM) could protect mouse peritoneal macrophages from oxidative injury. To uncover the mechanism further, we investigated the effect of L929-CM on the oxidative injury caused by tbOOH to RAW264.7 cell lines. The results showed that L929-CM could protect RAW264.7 cells from oxidative injury (presented by cell morphology and cell survival rate), and L929-CM could also improve total superoxide dismutase (SOD), selenium-dependent and non-selenium-dependent glutathione peroxidase (SeGPx and non-SeGPx) activities in RAW264.7 cells. RT-PCR analysis showed that, L929-CM could induce plasma glutathione peroxidase (PLGPx) mRNA expression, while there was no inducing effect of L929-CM on phospholipid hydroperoxide glutathione peroxidase (PHGPx) mRNA expression in RAW264.7 cells. 5μg/ml actinomycin D, 30μg/ml cycloheximide (de novo protein synthesis inhibitor) and 50μg/ml acetovanilone (intracellular superoxide anion production inhibitor) had no effects in attenuating the induction of PLGPx expression by L929-CM.