Polycyclic aromatic hydrocarbon degradation and biosurfactant production by a newly isolated Pseudomonas sp. strain from used motor oil-contaminated soil

• Published in: International biodeterioration & biodegradation Vol. 122; pp. 128 - 140
• Main Authors: Chebbi, Alif, Hentati, Dorra, Zaghden, Hatem, Baccar, Nidhal, Rezgui, Fatma, Chalbi, Manel, Sayadi, Sami, Chamkha, Mohamed
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.08.2017
• Subjects: Biodegradation; Biosurfactant; Hydrocarbon-remobilization; PAHs; Phenanthrene; Pseudomonas; Biosurfactant; Hydrocarbon-remobilization; Biodegradation; PAHs; Phenanthrene; Pseudomonas
• ISSN: 0964-8305; 1879-0208
• DOI: 10.1016/j.ibiod.2017.05.006

The aim of this study was to isolate and characterize a newly isolated bacterium, designated strain W10, a polycyclic aromatic hydrocarbon (PAHs) degrader and biosurfactant producer, belonged to Pseudomonas genus and closely related to Pseudomonas aeruginosa, with the 16 rRNA gene sequence similarity of 99.1%. Based on GC-MS analyses, it degraded around 80% of phenanthrene, used as the sole carbon and energy source, at an initial concentration of 200 mg l−1, after 30 days of incubation at 37 °C and 180 rpm, reducing the surface tension (ST) from 56.1 to 42 mN m−1 after 4 days of incubation. Furthermore, strain W10 utilized about 10%, 20%, 90%, and 99% of hexadecane (C16), pyrene, fluoranthene, and crude oil, respectively, after 30 days of incubation at 37 °C and 180 rpm. During the growth of strain W10 on phenanthrene and fluoranthene, some metabolites were identified, supporting the biodegradation pathways of the two PAHs. Interestingly, strain W10 showed also a significant potential to produce surface-active agents reducing the surface tension to 32 mN m−1 and reaching a production around 2 g l−1 after 48 h of incubation, in the presence of olive oil (1%,v/v) as substrate, at 37 °C and 180 rpm. Its biosurfactant, namely BSW10, showed an interesting emulsification activity and a high stability over a wide range of salinity (0–150 g l−1), temperature (0–100 °C), pH (2–12) and thus a promising abilities in used motor oil and crude oil removal from contaminated soils. Overall, these results provide evidence that strain W10 and its biosurfactant (BSW10) could be potential candidates for further bioremediation applications. [Display omitted] •A newly PAH degrader strain W10 belonging to Pseudomonas genus.•Interesting potential of degrading a wide range of hydrocarbons and oils.•Some metabolites of phenanthrene and fluoranthene biodegradation were detected.•The biosurfactant, BSW10, revealed stabilities against pH, temperature and salinity.•BSW10 revealed an interesting hydrocarbon remobilization on contaminated soils.