DUAL EFFECT OF IL-4 ON RESISTANCE TO SYSTEMIC GRAM-NEGATIVE INFECTION AND PRODUCTION OF TNF-α

• Published in: Cytokine (Philadelphia, Pa.) Vol. 12; no. 4; pp. 417 - 421
• Main Authors: Giampietri, A, Grohmann, U, Vacca, C, Fioretti, M.C, Puccetti, P, Campanile, F
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2000
• Subjects: Animals; Cells, Cultured; Disease Models, Animal; IL-4/IL-12/septic shock/TNF-α; Interleukin-12 - immunology; Interleukin-12 - pharmacology; Interleukin-4 - administration & dosage; Interleukin-4 - immunology; Interleukin-4 - metabolism; Lipopolysaccharides - immunology; Lipopolysaccharides - pharmacology; Macrophages - cytology; Macrophages - drug effects; Macrophages - immunology; Mice; Pseudomonas aeruginosa; Pseudomonas Infections - immunology; Pseudomonas Infections - prevention & control; Recombinant Proteins - administration & dosage; Recombinant Proteins - immunology; Recombinant Proteins - metabolism; Sepsis - immunology; Sepsis - prevention & control; Tumor Necrosis Factor-alpha - biosynthesis; IL-4/IL-12/septic shock/TNF-α
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1006/cyto.1999.0576

To determine the effect of interleukin 4 (IL-4) administration in a live sepsis model characterised by high-level production of tumour necrosis factor α (TNF-α), mice infected systemically with lethal or sublethal inocula of Pseudomonas aeruginosa were given the recombinant cytokine at different times before infection. Improved survival and decreased TNF-α production were observed in lethally infected mice treated with the cytokine 1 day before challenge. In contrast, increased mortality and overproduction of TNF-α were observed in sublethally infected mice given IL-4 at the time of infection.