Genomic characteristics of a novel strain Lactiplantibacillus plantarum X7021 isolated from the brine of stinky tofu for the application in food fermentation

• Published in: Food science & technology Vol. 156; p. 113054
• Main Authors: Liu, Guang, Liu, Yafan, Ro, Kum-Song, Du, Lei, Tang, Ya-Jie, Zhao, Li, Xie, Jingli, Wei, Dongzhi
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 15.02.2022
• Subjects: Comparative genomic analysis; Lactiplantibacillus plantarum; Nitrite degradation; Purine metabolism; β-glucosidase; Nitrite degradation; Comparative genomic analysis; β-glucosidase; Lactiplantibacillus plantarum; Purine metabolism
• ISSN: 0023-6438; 1096-1127
• DOI: 10.1016/j.lwt.2021.113054

Lactiplantibacillus plantarum X7021 is a novel strain originated from the brine of stinky tofu. In order to investigate the application potential of the strain in food industry, the genomic characteristics as well as some metabolic features were studied using comparison genomics and transcriptional assay. The complete genome of X7021includes a 3,407,054 bp chromosome with 3259 protein coding sequences, and 6 plasmids. L. plantarum strains, WCFS1, JDM1, and ST-III were selected for comparative genomic analysis. Results revealed that X7021 is safe for the application in foods with less antibiotic resistance and virulence genes. The strain has 25 complete phosphotransferase system transporters and strong proteolytic system for its adaptability to various foodstuffs. The strain containes a complete purine metabolic pathway incorporating 4 permeases, resulting in the reductions of 38.9% guanine, 26.1% hypoxanthine and 70.2% xanthine during the soymilk fermentation. Soy isoflavone glycosides could be converted to corresponding aglycones by two β-glucosidases. The strain could grow in 700 mg/L nitrite and entirely degrade 100 mg/L nitrite during 24 h based on highly active nitrite transporter and nitrite reductase. Accordingly, the results suggest that L. plantarum X7021 is a prospect starter candidate for the fermentation of plant foodstuffs. •Genomic features of L. plantarum X7021 showed its prospect in food fermentation.•X7021 possess strong viability based on its strong carbon and nitrogen utilization.•X7021 lower purine content in soymilk through its complete purine metabolic pathway.•X7021 can converse isoflavone glucoside to aglycone via two β-glucosidases.•Efficient expression of transporter & reductase for nitrite degradation.