slan/M-DC8+ cells constitute a distinct subset of dendritic cells in human tonsils
Human blood dendritic cells (DCs) include three main distinct subsets, namely the CD1c + and CD141 + myeloid DCs (mDCs) and the CD303 + plasmacytoid DCs (pDCs). More recently, a population of slan/M-DC8 + cells, also known as “slanDCs”, has been described in blood and detected even in inflamed secon...
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Published in | Oncotarget Vol. 7; no. 1; pp. 161 - 175 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Impact Journals LLC
05.01.2016
|
Subjects | |
Online Access | Get full text |
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Summary: | Human blood dendritic cells (DCs) include three main distinct subsets, namely the CD1c
+
and CD141
+
myeloid DCs (mDCs) and the CD303
+
plasmacytoid DCs (pDCs). More recently, a population of slan/M-DC8
+
cells, also known as “slanDCs”, has been described in blood and detected even in inflamed secondary lymphoid organs and non-lymphoid tissues. Nevertheless, hallmarks of slan/M-DC8
+
cells in tissues are poorly defined. Herein, we report a detailed characterization of the phenotype and function of slan/M-DC8
+
cells present in human tonsils. We found that tonsil slan/M-DC8
+
cells represent a unique DC cell population, distinct from their circulating counterpart and also from all other tonsil DC and monocyte/macrophage subsets. Phenotypically, slan/M-DC8
+
cells in tonsils display a CD11c
+
HLA-DR
+
CD14
+
CD11b
dim/neg
CD16
dim/neg
CX3CR1
dim/neg
marker repertoire, while functionally they exhibit an efficient antigen presentation capacity and a constitutive secretion of TNFα. Notably, such DC phenotype and functions are substantially reproduced by culturing blood slan/M-DC8
+
cells in tonsil-derived conditioned medium (TDCM), further supporting the hypothesis of a full DC-like differentiation program occurring within the tonsil microenvironment. Taken together, our data suggest that blood slan/M-DC8
+
cells are immediate precursors of a previously unrecognizedcompetent DC subset in tonsils, and pave the way for further characterization of slan/M-DC8
+
cells in other tissues. |
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ISSN: | 1949-2553 1949-2553 |
DOI: | 10.18632/oncotarget.6660 |