Separation of glycoprotein-derived oligosaccharides by thin-layer chromatography

A thin-layer chromatography (tlc) system has been developed for the separation of glycoprotein-derivedoligosaccharides. The method involves chromatography on silica gel using n-propanol/acetic acid/water (3:3:2 v/v) as the solvent. This tlc method was used to separate pathological oligosaccharides i...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 93; no. 1; pp. 167 - 170
Main Authors Holmes, Earle W., O'Brien, John S.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 1979
Subjects
Carbohydrates - analysis
Chromatography, Thin Layer - methods
G(M1) Ganglioside
Gangliosidoses - metabolism
Glycoproteins - metabolism
Humans
Liver - analysis
Molecular Conformation
Oligosaccharides - isolation & purification
NANA
GlcNAc
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Summary:A thin-layer chromatography (tlc) system has been developed for the separation of glycoprotein-derivedoligosaccharides. The method involves chromatography on silica gel using n-propanol/acetic acid/water (3:3:2 v/v) as the solvent. This tlc method was used to separate pathological oligosaccharides isolated from individuals with G M1 gangliosidosis and with neuraminidase deficiency. The results indicate the potential usefulness of the system in the analysis of complex carbohydrates.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0003-2697
1096-0309
DOI:10.1016/S0003-2697(79)80131-1