An efficient method for isolation and expansion of hematopoietic stem cells derived from human umbilical cord blood cells

• Published in: Biotechnology and bioprocess engineering Vol. 30; no. 2; pp. 336 - 344
• Main Authors: Park, Yo Seph, Ham, Seokbeom, Hwang, Hyunjoo, Hwang, Han Sung, Ko, Kisung, Lee, Tae Jin, Ko, Kinarm
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.04.2025; Springer Nature B.V; 한국생물공학회
• Subjects: Biotechnology; Blood; Blood cells; Cell therapy; Chemistry; Chemistry and Materials Science; Cord blood; Hematopoietic stem cells; humans; Industrial and Production Engineering; Medical research; Medicine; Methods; Peripheral blood; Reproducibility; Research Paper; researchers; Stem cells; Therapeutic applications; therapeutics; Transplants & implants; Umbilical cord; 생물공학; Functional assessment; Isolation of HSCs; Umbilical cord blood; Hematopoietic stem cells; Exo vivo expansion of HSCs
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-025-00191-0

Hematopoietic stem cells (HSCs) derived from human umbilical cord blood (UCB) have gained significant attention for cell therapy applications due to their numerous advantages. With increasing research interest in the isolation and application of UCB-derived HSCs, establishing standardized protocols for their isolation and functional assessment is essential. While the process of isolating HSCs from UCB shares similarities with peripheral blood procedures, variations in experimental conditions, such as researcher skill and equipment, can impact reproducibility. In this study, we present a method that minimizes experimental variables, ensuring consistent results in UCB-derived HSC isolation. Additionally, we detail protocols to verify the functionality of isolated HSCs. These standardized methods will aid researchers in reliably isolating and characterizing UCB-derived HSCs for both experimental and therapeutic applications.