DESI Mass Spectrometry Imaging (MSI)

Desorption Electrospray Ionization (DESI) mass spectrometry is a technique that allows chemical information to be obtained directly from a wide range of surfaces. Using a 2D stage, DESI can be implemented in an imaging mode whereby MS spectra are collected by rastering the spray across the whole sur...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1618; p. 65
Main Authors Claude, Emmanuelle, Jones, Emrys A, Pringle, Steven D
Format Journal Article
LanguageEnglish
Published United States 01.01.2017
Subjects
Lipids - chemistry
Mass Spectrometry - methods
Spectrometry, Mass, Electrospray Ionization - methods
DESI
Lipids
MSI
Imaging
MS
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Summary:Desorption Electrospray Ionization (DESI) mass spectrometry is a technique that allows chemical information to be obtained directly from a wide range of surfaces. Using a 2D stage, DESI can be implemented in an imaging mode whereby MS spectra are collected by rastering the spray across the whole surface. Here, we describe the implementation and optimization of DESI imaging for metabolites and lipids from tissue sections using oa-TOF mass spectrometers.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7051-3_7