Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac

Whole mount immunofluorescence is a valuable technique that can be used to visualize vascular networks in early developing embryonic tissues. This technique involves the permeabilization of fixed mouse embryos and yolk sacs, and primary antibody tagging of the endothelial cell marker platelet endoth...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1752; p. 11
Main Authors Roy, Anna R, Delgado-Olguin, Paul
Format Journal Article
LanguageEnglish
Published United States 01.01.2018
Subjects
Animals
Antibodies - metabolism
Embryo, Mammalian - blood supply
Endothelial Cells - metabolism
Female
Fluorescent Antibody Technique
Mice
Platelet Endothelial Cell Adhesion Molecule-1 - metabolism
Pregnancy
Yolk Sac - blood supply
Vascular network
Yolk sac
Embryo
Whole mount immunofluorescence
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Summary:Whole mount immunofluorescence is a valuable technique that can be used to visualize vascular networks in early developing embryonic tissues. This technique involves the permeabilization of fixed mouse embryos and yolk sacs, and primary antibody tagging of the endothelial cell marker platelet endothelial cell adhesion molecule 1 (Pecam-1). A secondary antibody tagged with a fluorophore targets the primary antibody, fluorescently labeling endothelial cells and revealing vascular networks.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7714-7_2