Co-administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 with prebiotic inulin alleviates the intestinal inflammation in rats exposed to N,N-dimethylhydrazine

• Published in: International immunopharmacology Vol. 24; no. 2; pp. 361 - 368
• Main Authors: Štofilová, Jana, Szabadosová, Viktória, Hrčková, Gabriela, Salaj, Rastislav, Bertková, Izabela, Hijová, Emília, Strojný, Ladislav, Bomba, Alojz
• Format: Journal Article
• Language: English
• Published: Netherlands 01.02.2015
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Carcinogens; Colon - drug effects; Colon - metabolism; Colon - pathology; Cyclooxygenase 2 - metabolism; Cytokines - metabolism; Dimethylhydrazines; Female; Inflammation - chemically induced; Inflammation - drug therapy; Inflammation - metabolism; Inflammation - pathology; Intestinal Diseases - drug therapy; Intestinal Diseases - metabolism; Intestinal Diseases - pathology; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Inulin - pharmacology; Inulin - therapeutic use; Jejunum - drug effects; Jejunum - metabolism; Lactobacillus plantarum; Male; NF-kappa B - metabolism; Nitric Oxide Synthase Type II - metabolism; Prebiotics; Probiotics - pharmacology; Probiotics - therapeutic use; Rats, Sprague-Dawley; Gut immunity; Cancer prevention; Flax-seed oil; Lactobacillus plantarum; Chronic inflammation; Inulin
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2014.12.022

The aim of this study was to determine the anti-inflammatory effects of preventive administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 alone or in combination with prebiotic inulin or with flax-seed oil in the gut of rats, which developed chronic inflammation following administration of the pro-carcinogen N,N-dimethylhydrazine (DMH). After 28weeks administration of probiotic/prebiotic-containing diet, rats were killed and their colons were examined by immunohistological criteria, whereas cytokines were determined in the jejunal mucosa. Application of DMH triggered the production of pro-inflammatory cytokines IL-2, IL-6, IL-17, and TNF-α, expression of pro-inflammatory mediators NF-κB, COX-2 and iNOS and caused depletion of goblet cells. Supplementing the diet with L. plantarum and its combination with the prebiotic abolished DMH-induced inflammatory process in the jejunal mucosa by inhibiting the production of pro-inflammatory cytokines and by stimulation of anti-inflammatory IL-10 cytokine synthesis, whereas concentration of TGF-β1 was not influenced significantly. Diet prevented a decrease in goblet cell numbers but numbers of mast cells were lowered only moderately. However, combined treatment of rats with L. plantarum and flax-seed oil had no significant effect on the parameters examined, except for decreased expression of NF-κB, in comparison with the negative control. Results indicate that the preventive administration of probiotic L. plantarum LS/07 CCM7766 alone or in combination with prebiotic inulin to rats with DMH-induced chronic inflammation can reduce inflammatory process in the jejunal and colon mucosa, probably indirectly, and involves down-regulation of synthesis of pro-inflammatory cytokines and suppression of NF-κB activity in mucosal cells.