Structural and phylogenetic analysis of α-glucosidase protein in insects

• Published in: Biológia Vol. 70; no. 6; pp. 812 - 825
• Main Authors: Seddigh, Samin, Darabi, Maryam
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.06.2015; De Gruyter Open
• Subjects: bioinformatics; Cell Biology; Life Sciences; Microbiology; phylogenetic tree; Plant Sciences; primary structure; tertiary structure; Zoology; α-glucosidase; tertiary structure; phylogenetic tree; glucosidase; primary structure; bioinformatics
• ISSN: 0006-3088; 1336-9563
• DOI: 10.1515/biolog-2015-0096

α -Glucosidases ( α glus) (EC 3.2.1.20) have been detected in a wide range of plants, animals and microorganisms and are described by their ability to catalyze the hydrolysis of 1,4- α -glucosidic linkages, releasing α -glucose. In this study, 96 α glu protein sequences from 33 organisms species including different insects species, plants (including thale cress and rice), mammals (including human and mouse) and Mycobacterium tuberculosis as a bacterium were aligned. Sequences were analyzed by computational tools to predict the protein properties, such as molecular mass, isoelectric point, signal peptide, conserved motifs, transmembrane domain and secondary structure. Drosophila melanogaster (GenBank Accession No.: NP 610382) was chosen as one of the representatives of insects for further analyses. The tertiary structure of representative samples were acquired using the tertiary structure of oligo-1,6-glucosidase from Bacillus cereus (Protein Data Bank code: 1UOK) as a template by Phyre2 server. Protein structure analysis revealed there is a high identity among insects and other organisms. There were some similar functional domains between D. melanogaster and M. tuberculosis . The modeled α glu has a typical spatial structure in insects and exhibits a high similarity with other organisms, especially Arabidopsis thaliana. Phylogenetic analysis indicated that D. melanogaster α glu has a close relationship with other α glus from different insect families. According to these results, α glu in insects should be evolved from a common ancestor.