Purification and Characterization of the Bacterial Flagellar Basal Body from Salmonella enterica

The bacterial flagellum is a motility organelle. The flagellum is composed of three main structures: the basal body as a rotary engine embedded in the cellular membranes and cell wall, the long external filament that acts as a propeller, and the hook acting as a universal joint that connects them. I...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1593; p. 87
Main Author Aizawa, Shin-Ichi
Format Journal Article
LanguageEnglish
Published United States 2017
Subjects
Bacterial Proteins - metabolism
Basal Bodies - metabolism
Cell Membrane Structures - metabolism
Flagella - metabolism
Salmonella typhimurium - metabolism
Hook
Filament
Lipopolysaccharide
Cytoplasmic membrane
Electron microscopy
Peptidoglycan layer
Basal body
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Summary:The bacterial flagellum is a motility organelle. The flagellum is composed of three main structures: the basal body as a rotary engine embedded in the cellular membranes and cell wall, the long external filament that acts as a propeller, and the hook acting as a universal joint that connects them. I describe protocols for the purification of the filament and hook-basal body from Salmonella enterica serovar Typhimurium.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6927-2_6