A ROLE FOR POTASSIUM IN TNF-INDUCED APOPTOSIS AND GENE-INDUCTION IN HUMAN AND RODENT TUMOUR CELL LINES

Rat/mouse T cell hybridoma-derived PC60 R55/R75 cells were used as a model to study the role of intracellular potassium in TNF-induced apoptosis and gene induction. A reduction of intracellular potassium with nigericin or valinomycin (ionophores), or ouabain (Na+/K+-ATPase inhibitor) sensitized PC60...

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Published inCytokine (Philadelphia, Pa.) Vol. 12; no. 6; pp. 747 - 750
Main Authors Penning, Louis C, Denecker, Geertrui, Vercammen, Dominique, Declercq, Wim, Schipper, Raymond G, Vandenabeele, Peter
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.06.2000
Subjects
Animals
Apoptosis - drug effects
Apoptosis - physiology
apoptosis/GM-CSF/potassium/T cell hybridoma/TNF
Cell Survival - drug effects
Gene Expression Regulation, Neoplastic - drug effects
Gene Expression Regulation, Neoplastic - physiology
Granulocyte-Macrophage Colony-Stimulating Factor - genetics
HeLa Cells
Humans
Hybridomas
Mice
Nigericin - pharmacology
Ouabain - pharmacology
Potassium - physiology
Recombinant Proteins - pharmacology
T-Lymphocytes
Transcriptional Activation
Tumor Necrosis Factor-alpha - pharmacology
Valinomycin - pharmacology
apoptosis/GM-CSF/potassium/T cell hybridoma/TNF
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Summary:Rat/mouse T cell hybridoma-derived PC60 R55/R75 cells were used as a model to study the role of intracellular potassium in TNF-induced apoptosis and gene induction. A reduction of intracellular potassium with nigericin or valinomycin (ionophores), or ouabain (Na+/K+-ATPase inhibitor) sensitized PC60 R55/R75 cells to TNF-induced apoptosis. TNF-induced GM-CSF release in PC60 R55/R75 cells was enhanced by nigericin or ouabain. Similar results were obtained with human cervix carcinoma cells HeLaH21 exposed to TNF. These results suggest a role for intracellular potassium in TNF-induced apoptosis and gene induction.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1043-4666
1096-0023
DOI:10.1006/cyto.1999.0626