Increasing N,N-dimethylacetamide degradation and mineralization efficiency by co-culture of Rhodococcus ruber HJM-8 and Paracoccus communis YBH-X

• Published in: Chemosphere (Oxford) Vol. 303; no. Pt 1; p. 134935
• Main Authors: Yuan, Bohan, Yao, Jiachao, Wang, Zeyu, Dai, Luyao, Zhao, Min, Hrynsphan, Dzmitry, Tatsiana, Savitskaya, Chen, Jun
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2022
• Subjects: Acetate; acetates; Biodegradation; Co-culture; coculture; DMAC; electron transfer; experimental design; microbial activity; Mineralization; polymers; Rhodococcus ruber; Biodegradation; DMAC; Acetate; Co-culture; Mineralization
• ISSN: 0045-6535; 1879-1298; 1879-1298
• DOI: 10.1016/j.chemosphere.2022.134935

In this work, Rhodococcus ruber HJM-8 and Paracoccus communis YBH-X were isolated and used to enhance N,N-dimethylacetamide (DMAC) degradation and mineralization efficiencies. The monoculture and co-culture of the two strains for DMAC degradation were compared; results indicated that, a degradation efficiency of 97.62% was obtained in co-culture, which was much higher than that of monocultures of HJM-8 (57.34%) and YBH-X (34.02%). The degradation mechanism showed that co-culture could efficiently improve extracellular polymeric substances production, electron transfer, and microbial activity. Meanwhile, the mineralization mechanism suggested that acetate was the dominant intermediate which had an inhibitory effect on HJM-8, and co-culture was conducive to mineralization due to the high performance of acetate conversion and Na+ K+-ATPase vitality. Besides, a pathway of DMAC biodegradation was proposed for co-culture: DMAC was degraded into acetate by HJM-8, then the accumulated acetate was mineralized by YBH-X. Additionally, the co-culture system was further optimized by Box-Behnken design. [Display omitted] •Rhodococcus ruber HJM-8 and Paracoccus communis YBH-X were isolated and used for DMAC biodegradation.•DMAC degradation and mineralization efficiencies could be efficiently enhanced by co-culture.•DMAC degradation and mineralization mechanism in co-culture system were exhibited.•Box-Behnken design was applied for optimization of the co-culture system.