Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling

To better understand and model neurological, in particular neurodegenerative diseases, human induced pluripotent stem cells (hiPSCs) offer a great source for generation of neural cells. We provide a protocol for the differentiation of hiPSc-derived astrocytes in vitro. This protocol not only is chem...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1994; p. 31
Main Authors Janssen, Katharina, Bahnassawy, Lamiaa, Kiefer, Claudia, Korffmann, Jürgen, Terstappen, Georg C, Lakics, Viktor, Cik, Miroslav, Reinhardt, Peter
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Astrocytes - cytology
Cell Culture Techniques
Cell Differentiation - drug effects
Cells, Cultured
Culture Media
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - drug effects
Models, Biological
Neural Stem Cells - cytology
Neural Stem Cells - drug effects
Neurodegenerative Diseases
Neurogenesis - drug effects
Neurodegenerative disease
Disease modeling
Astrocytes
hiPSC
In vitro
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Summary:To better understand and model neurological, in particular neurodegenerative diseases, human induced pluripotent stem cells (hiPSCs) offer a great source for generation of neural cells. We provide a protocol for the differentiation of hiPSc-derived astrocytes in vitro. This protocol not only is chemically defined, that is, it does not use serum, but also allows for the expansion of astrocyte progenitor cells and mature astrocytes. Large batches of hiPSc-derived astrocytes can be stored and used for defined in vitro disease models.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9477-9_3