Co-localization of neuronal nitric oxide synthase with arginine–vasotocin in the preoptic–hypothalamo–hypophyseal system of the teleost Oreochromis niloticus

• Published in: Brain research Vol. 1015; no. 1; pp. 181 - 185
• Main Authors: Bordieri, Loredana, Cioni, Carla
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 23.07.2004; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Biological and medical sciences; Cichlids - anatomy & histology; Cichlids - metabolism; Co-expression; Confocal laser scanning microscopy; Double labeling immunocytochemistry; Female; Fish; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Hypothalamo-Hypophyseal System - cytology; Hypothalamo-Hypophyseal System - metabolism; Hypothalamus. Hypophysis. Epiphysis. Urophysis; Immunohistochemistry; Male; Morphology. Functional localizations; Neuroendocrine system; Neurons - cytology; Neurons - enzymology; Neurons - metabolism; Nitric Oxide Synthase - metabolism; Nitric Oxide Synthase Type I; NOS activity; Oreochromis niloticus; Preoptic Area - cytology; Preoptic Area - metabolism; Subcellular Fractions - metabolism; Tissue Distribution; Tyramide signal amplification; Vasotocin - metabolism; Vertebrates: endocrinology; NOS activity; Endocrine and autonomic regulation; Fish; Co-expression; Tyramide signal amplification; Double labeling immunocytochemistry; Neuroendocrine system; Confocal laser scanning microscopy; Enzyme; Central nervous system; Confocal microscopy; Hypothalamus; Nitric-oxide synthase; Encephalon; Vertebrata; Oreochromis niloticus; Arginine; Pisces; Preoptic nucleus; Pituitary gland; Vasotocin; Oxidoreductases; Localization
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2004.04.059

This study provides evidence that, in the preoptic–hypothalamo–hypophysial system of the teleost Oreochromis niloticus, several sub-populations of arginine–vasotocin (AVT)-producing neurons and neurosecretory fibers terminals express neuronal nitric oxide synthase (nNOS)-like molecules. The co-localization between nNOS and AVT was demonstrated by means of double immunofluorescence staining with the confocal microscope. This study is the first to provide evidence that nNOS may be co-localized with AVT in neurons of a non-mammalian vertebrate.