SPECTROSCOPIC AND KINETIC STUDIES OF INTERACTIONS OF CALF SPLEEN PURINE NUCLEOSIDE PHOSPHORYLASE WITH 8-AZAGUANINE, AND ITS 9-(2-PHOSPHONYLMETHOXYETHYL) DERIVATIVE

Spectroscopic and kinetic studies of interactions of calf spleen purine nucleoside phosphorylase with 8-azaguanine, an excellent fluorescent/fluorogenic substrate for the synthetic pathway of the reaction, and its 9-(2-phosphonylmethoxyethyl) derivative, a bisubstrate analogue inhibitor, were carrie...

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Published inNucleosides, nucleotides & nucleic acids Vol. 24; no. 5-7; pp. 459 - 464
Main Authors Wierzchowski, Jacek, Stępniak, Katarzyna, Bzowska, Agnieszka, Shugar, David
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis Group 01.01.2005
Subjects
8-Azaguanine
Animals
Antimetabolites, Antineoplastic - pharmacology
Azaguanine - chemistry
Azaguanine - pharmacology
Catalytic Mechanism
Cattle
Drug Interactions
Enzyme-Ligand Complexes
Fluorescence
Hydrogen-Ion Concentration
Kinetics
Ligands
Macromolecular Substances - chemistry
Models, Chemical
Purine Nucleoside Phosphorylase
Purine-Nucleoside Phosphorylase - chemistry
Spectrometry, Fluorescence - methods
Spleen - enzymology
Substrate Specificity
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Summary:Spectroscopic and kinetic studies of interactions of calf spleen purine nucleoside phosphorylase with 8-azaguanine, an excellent fluorescent/fluorogenic substrate for the synthetic pathway of the reaction, and its 9-(2-phosphonylmethoxyethyl) derivative, a bisubstrate analogue inhibitor, were carried out. The goal was to clarify the catalytic mechanism of the enzymatic reaction by identification of ionic/tautomeric forms of these ligands in the complex with PNP.
ISSN:1525-7770
1532-2335
DOI:10.1081/NCN-200060004