DNA amplification fingerprinting identifies closely related Chrysanthemum cultivars

DNA amplification fingerprinting (DAF) was used to study genetic relationships between closely related chrysanthemum cultivars (Dendranthema grandiflora Tzvelev.). Twenty-one cultivars were examined that belonged to the Anne, Blush, Boaldi, Charm, Davis, and Pomona series (families). The genetic var...

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Bibliographic Details
Published inJournal of the American Society for Horticultural Science Vol. 121; no. 6; pp. 1043 - 1048
Main Authors Scott, M.C. (The University of Tennessee, Knoxville, TN.), Caetano-Anolles, G, Trigiano, R.N
Format Journal Article
LanguageEnglish
Published 01.11.1996
Subjects
ADN
ANATOMIA DE LA PLANTA
ANATOMIE VEGETALE
CLASIFICACION
CLASSIFICATION
DENDRANTHEMA
DENDRANTHEMA GRANDIFLORA
DNA
DNA FINGERPRINTING
EMPREINTE ADN
GENETIC POLYMORPHISM
GENETIC VARIATION
HUELLAS GENETICAS ADN
INFLORESCENCE
INFLORESCENCES
INFLORESCENCIAS
MOLECULAR SEQUENCE DATA
NUCLEOTIDE SEQUENCE
OHIO
PHENETICS
PLANT ANATOMY
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
VARIACION GENETICA
VARIATION GENETIQUE
VARIEDADES
VARIETE
VARIETIES
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Summary:DNA amplification fingerprinting (DAF) was used to study genetic relationships between closely related chrysanthemum cultivars (Dendranthema grandiflora Tzvelev.). Twenty-one cultivars were examined that belonged to the Anne, Blush, Boaldi, Charm, Davis, and Pomona series (families). The genetic variability of cultivars within and between series was evaluated using eleven arbitrary octamer primers. A few polymorphic characters uniquely identified closely related cultivars within each of the series. In contrast, many DNA polymorphisms were observed between members of the different series. Phenetic patterns were established by unweighted pair group cluster analysis using arithmetic means (UPGMA) and principal coordinate analysis (PCO). The average distance between series was 10-fold greater than between cultivars within a series. DNA from all cultivars belonging to a series were also bulked to generate profiles containing unique amplified products for each series. Cluster analysis and PCO of bulked DNA clearly grouped Charm and Pomona together. However, series grouping did not correspond to morphology of inflorescence types. The results demonstrate the utility of the DAF technique in distinguishing clonal materials and its potential use for patent protection, phylogenetic studies, and for identifying useful markers in breeding applications
Bibliography:1997056157
F30
ISSN:0003-1062
2327-9788
DOI:10.21273/JASHS.121.6.1043