Renibacterium salmoninarum isolates from different sources possess two highly conserved copies of the rRNA operon

• Published in: Antonie van Leeuwenhoek Vol. 78; no. 1; pp. 51 - 61
• Main Authors: GRAYSON, T. Hilton, ALEXANDER, Sarah M, COOPER, Lynne F, GILPIN, Martyn L
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.07.2000; Springer Nature B.V
• Subjects: Actinomycetales - genetics; Bacteria; Bacteriology; Base Sequence; Biological and medical sciences; Conserved Sequence; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Gene Dosage; Genetic Variation; Genetics; Gram-Positive Asporogenous Rods, Regular - genetics; Microbiology; Molecular Sequence Data; Operon; Phylogeny; Polymerase Chain Reaction; Renibacterium salmoninarum; Restriction Mapping; RNA, Ribosomal - genetics; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 23S - genetics; RNA, Ribosomal, 5S - genetics; rRNA; rRNA operon; Sequence Analysis, DNA; Genetic variability; Nucleotide sequence; Operon; Ribosomal DNA; 16S-RNA; Bacteria; Homology; Spacer DNA; 23S-RNA; 5S-RNA; Renibacterium salmoninarum
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1023/A:1002745129625

The nucleotide sequences of the rRNA genes and the 5' flanking region were determined for R. salmoninarum ATCC 33209T from overlapping products generated by PCR amplification from the genomic DNA. Comparison of the sequences with rRNA genes from a variety of bacteria demonstrated the close relatedness between R. salmoninarum and the high G+C group of the actinobacteria, in particular, Arthrobacter species. A regulatory element within the 5' leader of the rRNA operon was identical to an element, CL2, described for mycobacteria. PCR, DNA sequence analysis, and DNA hybridisation were performed to examine variation between isolates from diverse sources which represented the four 16S-23S rRNA intergenic spacer sequevars previously described for R. salmoninarum. Two 23S-5S rRNA intergenic spacer sequevars of identical length were found. DNA hybridisation using probes complementary to 23S rDNA and 16S rDNA identified two rRNA operons which were identical or nearly identical amongst 40 isolates sourced from a variety of countries.