Use of antisera against bovine (NCDV) and simian (SA11) rotaviruses in ELISA to detect different types of human rotavirus

Two ELISA systems for the detection of human rotaviruses were developed. In the first system antibodies to Nebraska calf diarrhea virus (NCDV) were used for coating the solid matrix and for the preparation of the enzyme conjugate. In the second system antibodies to human rotavirus and antibodies to...

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Bibliographic Details
Published inCanadian journal of microbiology Vol. 25; no. 9; p. 1118
Main Authors Bishai, F R, Spence, L, Goodwin, D, Petro, R
Format Journal Article
LanguageEnglish
Published Canada 01.09.1979
Subjects
Animals
Antigens, Viral - analysis
Cattle
Counterimmunoelectrophoresis
Enzyme-Linked Immunosorbent Assay
Epitopes
Feces - microbiology
Haplorhini
Hemagglutination Tests
Humans
Immune Sera
Immunoenzyme Techniques
Microscopy, Electron
RNA Viruses - isolation & purification
Rotavirus - immunology
Rotavirus - isolation & purification
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Summary:Two ELISA systems for the detection of human rotaviruses were developed. In the first system antibodies to Nebraska calf diarrhea virus (NCDV) were used for coating the solid matrix and for the preparation of the enzyme conjugate. In the second system antibodies to human rotavirus and antibodies to simian rotavirus (SA11) were used for coating the solid matrix and for the preparation of the enzyme conjugate respectively. The second ELISA system proved to have a broader spectrum for the detection of human rotaviruses. By using the two ELISA systems, the different types of human rotavirus could be distinguished. The ELISA tests developed were 8 to 64 times as sensitive as electron microscopy (EM) and (or) counterimmunoelectrophoresis (CIEP). The antigen detected by ELISA was shown to be different from that detected by the hemagglutination test.
ISSN:0008-4166
DOI:10.1139/m79-174