Immunohistochemical Characterization of Lymphosarcoma in Two Alpacas ( Lama pacos)

• Published in: Journal of comparative pathology Vol. 127; no. 1; pp. 69 - 71
• Main Authors: Hemsley, S., Bailey, G., Canfield, P.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2002
• Subjects: Animals; Antigens, CD - analysis; Antigens, Neoplasm - analysis; B-Lymphocytes - chemistry; B-Lymphocytes - pathology; Biomarkers, Tumor - analysis; Camelids, New World; Cross Reactions - immunology; Fatal Outcome; Female; Humans; Immunoenzyme Techniques - veterinary; Immunohistochemistry - veterinary; Immunophenotyping; Lymph Nodes - chemistry; Lymph Nodes - pathology; Lymphoma, Non-Hodgkin - chemistry; Lymphoma, Non-Hodgkin - immunology; Lymphoma, Non-Hodgkin - pathology; Lymphoma, Non-Hodgkin - veterinary; Male; T-Lymphocytes - chemistry; T-Lymphocytes - pathology
• ISSN: 0021-9975; 1532-3129
• DOI: 10.1053/jcpa.2002.0555

Species cross-reactive anti-peptide antibodies were assessed in formalin-fixed tissue for use in immunophenotyping of lymphosarcoma in two alpacas. Diagnosis of lymphosarcoma was made by routine histopathological examination. Primary antibodies used for immunophenotyping were anti-human CD3 and anti-human CD5 for T cells; and anti-human CD79a and anti-human CD79b for B cells/plasma cells. In one case, most of the neoplastic cells were labelled with both anti-CD3 and anti-CD79b, and smaller numbers were labelled with anti-CD79a. The other case was classified as a B-cell tumour on the basis of labelling of the majority of neoplastic cells with anti-CD79b and anti-CD79a. This is the first recorded attempt at immunophenotyping lymphosarcoma in alpacas and, to our knowledge, the first record of presumptive co-expression of T- and B-cell-associated molecules in lymphosarcoma in the veterinary literature.