A Comparison of Lyophilized Amniotic Membrane with Cryopreserved Amniotic Membrane for the Reconstruction of Rabbit Corneal Epithelium

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to suppor...

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Published inBiotechnology and bioprocess engineering Vol. 10; no. 3; pp. 262 - 269
Main Authors Ahn, J.I. (Dongguk University, Seoul, Republic of Korea), Jang, I.K. (Dongguk University, Seoul, Republic of Korea), Lee, D.H. (Dongguk University, Seoul, Republic of Korea), Seo, Y.K. (Dongguk University, Seoul, Republic of Korea), Yoon, H.H. (Dongguk University, Seoul, Republic of Korea), Shin, Y.H. (Dongguk University, Seoul, Republic of Korea), Kim, J.C. (Chung-Ang University Hospital, Seoul, Republic of Korea), Song, K.Y. (Chung-Ang University, Seoul, Republic of Korea), Lee, H.G. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Yang, E.K. (Bioland Ltd., Cheonan, Republic of Korea), Kim, K.H. (Bioland Ltd., Cheonan, Republic of Korea), Park, J.K. (Dongguk University, Seoul, Republic of Korea), E-mail: jungkeug@empal.com
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.06.2005
Subjects
Amniotic membrane
Animal models
Cornea
Cryopreservation
EPITELIO
Epithelial cells
EPITHELIUM
EYES
Gamma irradiation
gamma Radiation
Irradiation
Membranes
OEIL
OJOS
Polytetrafluoroethylene
Reconstruction
Shelf life
teflon ring
Transplantation
Transplants & implants
γ Radiation
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Summary:Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an explantation method. The corneal epithelium could be reconstructed by culturing the third-­passage corneal epithelial cells on the AM.
Bibliography:E21
2006016637
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ISSN:1226-8372
1976-3816
DOI:10.1007/BF02932023