MALDI-MSI of Lipids in Human Skin
Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is now a well-established technique for imaging analysis of sectioned biological tissues. One of the growing areas of interest is in the analysis of skin. MALDI-MSI can provide a wealth of information from within sections of...
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Published in | Methods in molecular biology (Clifton, N.J.) Vol. 1618; p. 29 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
01.01.2017
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Abstract | Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is now a well-established technique for imaging analysis of sectioned biological tissues. One of the growing areas of interest is in the analysis of skin. MALDI-MSI can provide a wealth of information from within sections of skin. This includes information on the distribution of pharmaceuticals following topical treatments, through to the examination of the composition of different skin layers and studies of proteomic, lipidomic, and metabolomic responses to disease, wounds, and external stimuli. Here, we describe the handling procedures, preparatory treatment, and mass spectrometry setup required for the MALDI MSI analysis of lipids within human skin samples. |
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AbstractList | Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is now a well-established technique for imaging analysis of sectioned biological tissues. One of the growing areas of interest is in the analysis of skin. MALDI-MSI can provide a wealth of information from within sections of skin. This includes information on the distribution of pharmaceuticals following topical treatments, through to the examination of the composition of different skin layers and studies of proteomic, lipidomic, and metabolomic responses to disease, wounds, and external stimuli. Here, we describe the handling procedures, preparatory treatment, and mass spectrometry setup required for the MALDI MSI analysis of lipids within human skin samples. |
Author | Clench, Malcolm R Hart, Philippa J |
Author_xml | – sequence: 1 givenname: Philippa J surname: Hart fullname: Hart, Philippa J email: Philippa_hart@waters.com organization: Waters Corporation, Stamford Ave., Altrincham Road, Wilmslow, SK9 4AX, UK. Philippa_hart@waters.com – sequence: 2 givenname: Malcolm R surname: Clench fullname: Clench, Malcolm R organization: Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28523497$$D View this record in MEDLINE/PubMed |
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SubjectTerms | Humans Lipids - analysis Proteomics - methods Skin - metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods |
Title | MALDI-MSI of Lipids in Human Skin |
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