MALDI-MSI of Lipids in Human Skin

Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is now a well-established technique for imaging analysis of sectioned biological tissues. One of the growing areas of interest is in the analysis of skin. MALDI-MSI can provide a wealth of information from within sections of...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1618; p. 29
Main Authors Hart, Philippa J, Clench, Malcolm R
Format Journal Article
LanguageEnglish
Published United States 01.01.2017
Subjects
Humans
Lipids - analysis
Proteomics - methods
Skin - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Lipids
MSI
Skin
Imaging
MALDI
MS
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Summary:Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is now a well-established technique for imaging analysis of sectioned biological tissues. One of the growing areas of interest is in the analysis of skin. MALDI-MSI can provide a wealth of information from within sections of skin. This includes information on the distribution of pharmaceuticals following topical treatments, through to the examination of the composition of different skin layers and studies of proteomic, lipidomic, and metabolomic responses to disease, wounds, and external stimuli. Here, we describe the handling procedures, preparatory treatment, and mass spectrometry setup required for the MALDI MSI analysis of lipids within human skin samples.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7051-3_4