Development of 27 trinucleotide microsatellite markers for Saccharina japonica using next generation sequencing technology
Twenty-seven polymorphic trinucleotide microsatellite DNA markers were developed from Saccharina japonica, through paired-end Illumina sequencing data. Sixty-three gametophyte clones of the species, preserved indoor as germplasm resources were genotyped using these markers. The number of alleles ran...
Saved in:
Published in | Conservation genetics resources Vol. 6; no. 2; pp. 341 - 344 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer-Verlag
01.06.2014
Springer Netherlands Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Twenty-seven polymorphic trinucleotide microsatellite DNA markers were developed from Saccharina japonica, through paired-end Illumina sequencing data. Sixty-three gametophyte clones of the species, preserved indoor as germplasm resources were genotyped using these markers. The number of alleles ranged from 2 to 7 with an average 4.7 per locus. The gene diversity and expected heterozygosity varied from 0.171 to 0.774 and from 0.174 to 0.793, respectively. Sixteen loci could be successfully amplified in Saccharina longissima with 12 loci being polymorphic. These novel microsatellite DNA markers will be extremely useful in germplasm resource conservation and management of S. japonica and also in its related species S. longissima. |
---|---|
Bibliography: | http://dx.doi.org/10.1007/s12686-013-0089-0 |
ISSN: | 1877-7252 1877-7260 |
DOI: | 10.1007/s12686-013-0089-0 |