Development of 27 trinucleotide microsatellite markers for Saccharina japonica using next generation sequencing technology

• Published in: Conservation genetics resources Vol. 6; no. 2; pp. 341 - 344
• Main Authors: Zhang, Linan, Peng, Jie, Li, Xiaojie, Liu, Yanling, Cui, Cuiju, Wu, Hao, Wu, Ruina, Tian, Pingping, Li, Yan
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.06.2014; Springer Netherlands; Springer Nature B.V
• Subjects: alleles; Animal Genetics and Genomics; Biodiversity; Biomedical and Life Sciences; clones; Conservation Biology/Ecology; Deoxyribonucleic acid; DNA; DNA sequencing; Ecology; Evolutionary Biology; gametophytes; Genetic markers; Germplasm; Heterozygosity; Life Sciences; loci; Microsatellite Letters; microsatellite repeats; Microsatellites; Next-generation sequencing; Plant Genetics and Genomics; Saccharina japonica; Microsatellite; Next generation DNA sequencing
• ISSN: 1877-7252; 1877-7260
• DOI: 10.1007/s12686-013-0089-0

Twenty-seven polymorphic trinucleotide microsatellite DNA markers were developed from Saccharina japonica, through paired-end Illumina sequencing data. Sixty-three gametophyte clones of the species, preserved indoor as germplasm resources were genotyped using these markers. The number of alleles ranged from 2 to 7 with an average 4.7 per locus. The gene diversity and expected heterozygosity varied from 0.171 to 0.774 and from 0.174 to 0.793, respectively. Sixteen loci could be successfully amplified in Saccharina longissima with 12 loci being polymorphic. These novel microsatellite DNA markers will be extremely useful in germplasm resource conservation and management of S. japonica and also in its related species S. longissima.