In Vivo Imaging of Budding Yeast Meiosis

Tracking biological events in living cells provides kinetic information about biological processes that can be missed in more traditional methods using fixed samples at designated time intervals. Here we describe a methodology for in vivo fluorescence microscopy of yeast cells undergoing meiosis. Th...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1471; p. 175
Main Authors Pollard, Michael G, Fung, Jennifer C
Format Journal Article
LanguageEnglish
Published United States 01.01.2017
Subjects
Meiosis
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Molecular Imaging - methods
Saccharomycetales - genetics
Saccharomycetales - physiology
Meiosis
Yeast
Live imaging
In vivo fluorescence microscopy
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Summary:Tracking biological events in living cells provides kinetic information about biological processes that can be missed in more traditional methods using fixed samples at designated time intervals. Here we describe a methodology for in vivo fluorescence microscopy of yeast cells undergoing meiosis. This method allows tracking of individual cells over extended periods of time through every stage of the meiotic transformation while minimizing phototoxicity and sustaining conditions that support meiotic growth.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6340-9_9