Role of the Ly 1 antigen in interleukin 1-induced thymocyte activation

Interleukin 1 (IL 1), but not IL 2-induced thymocyte proliferation was augmented by monoclonal antibodies (mAb) against the Ly 1 antigen. The anti-Ly 1 mAb could also co-stimulate thymocytes with phytohemagglutinin (PHA) alone, mimicking the effect of IL 1. Moreover, overnight culture with PHA of th...

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Bibliographic Details
Published inEuropean journal of immunology Vol. 15; no. 10; p. 1007
Main Authors Lögdberg, L, Shevach, E M
Format Journal Article
LanguageEnglish
Published Germany 1985
Subjects
Animals
Antibodies, Monoclonal - immunology
Antigens, Ly - immunology
Biological Assay
Cells, Cultured
Hybridomas - immunology
Interleukin-1 - analysis
Interleukin-1 - immunology
Interleukin-2 - biosynthesis
Isoantibodies - immunology
Lymphocyte Activation
Mice
Mice, Inbred Strains
Phytohemagglutinins - immunology
T-Lymphocytes - immunology
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Summary:Interleukin 1 (IL 1), but not IL 2-induced thymocyte proliferation was augmented by monoclonal antibodies (mAb) against the Ly 1 antigen. The anti-Ly 1 mAb could also co-stimulate thymocytes with phytohemagglutinin (PHA) alone, mimicking the effect of IL 1. Moreover, overnight culture with PHA of thymocytes, but not of spleen T lymphocytes led to selectively enhanced expression of the Ly 1 antigen on the cell surface. Similar data were generated at the clonal level. Thus, a thymocyte hybridoma (22A6) could be stimulated to release IL 2 in response to PHA plus IL 1, but also to PHA plus anti-Ly 1 mAb. In addition, 22A6 exhibited enhanced cell surface expression of the Ly 1 antigen after overnight culture with PHA. These data suggest a critical role for the Ly 1 antigen in thymocyte proliferation, perhaps by serving as an IL 1-receptor.
ISSN:0014-2980
DOI:10.1002/eji.1830151009