Mapping Quantitative Trait Loci for Powdery Mildew Resistance in Flax (Linum usitatissimum L.)

ABSTRACT Powdery mildew (Oidium lini Skoric, [PM]), an obligate biotrophic ascomycete, is a common and widespread foliar disease of flax in most flax‐growing areas of the world. In this study, we constructed a linkage map using 143 simple sequence repeat (SSR) markers and an F2 population of 300 ind...

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Published inCrop science Vol. 53; no. 6; pp. 2462 - 2472
Main Authors Asgarinia, Parvaneh, Cloutier, Sylvie, Duguid, Scott, Rashid, Khalid, Mirlohi, AghaFakhr, Banik, Mitali, Saeidi, Ghodratollah
Format Journal Article
LanguageEnglish
Published Madison The Crop Science Society of America, Inc 01.11.2013
American Society of Agronomy
Subjects
Airborne microorganisms
Cloning
Cultivars
Gene loci
Gene mapping
Genetics
Genomes
Genomics
Genotype & phenotype
Growth chambers
Phenotypic variations
Seeds
China
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Summary:ABSTRACT Powdery mildew (Oidium lini Skoric, [PM]), an obligate biotrophic ascomycete, is a common and widespread foliar disease of flax in most flax‐growing areas of the world. In this study, we constructed a linkage map using 143 simple sequence repeat (SSR) markers and an F2 population of 300 individuals generated from a cross between the susceptible cultivar NorMan and the resistant cultivar Linda. The F2–derived F3 families were phenotyped in the field and the F3–derived F4 families were phenotyped in a controlled environment growth chamber. The 15 linkage group map covered 1241 cM and was largely collinear with the previously published consensus map. Quantitative trait loci (QTL) analysis was performed and three PM resistance QTL located on LG1, 7, and 9 were identified consistently using phenotyping data from both field and growth chamber studies. These QTL explained 97% of the phenotypic variation exhibiting a mainly dominant gene action. This work represents a first step toward understanding the genetics of PM resistance in flax and map‐based cloning of candidate genes underlying the QTL.
Bibliography:All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.
This research was supported by Isfahan University of Technology (IUT), Iranian Ministry of Science, Research and Technology and an Agriculture and Agri‐Food Canada A‐base grant.
ISSN:0011-183X
1435-0653
DOI:10.2135/cropsci2013.05.0298