Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis

• Published in: Chinese medical sciences journal Vol. 31; no. 4; pp. 207 - 212
• Main Authors: Si, Hai-bo, Zeng, Yi, Zhou, Zong-ke, Pei, Fu-xing, Lu, Yan-rong, Cheng, Jing-qiu, Shen, Bin
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.12.2016; Department of 0rthopedics, Regenerative Medicine Research Centre, West China Hospital, Sichuan University, Chengdu 610041, China%Key Laboratory of Transplant Engineering and Immunology, Regenerative Medicine Research Centre, West China Hospital, Sichuan University, Chengdu 610041, China
• Subjects: Adult; Aged; Cells, Cultured; chondrocytes; Chondrocytes - metabolism; Female; fluid; Humans; Knee Joint - metabolism; Male; microRNA-140; MicroRNAs - analysis; Middle Aged; osteoarthritis; Osteoarthritis, Knee - metabolism; Real-Time Polymerase Chain Reaction; synovial; synovial fluid; Synovial Fluid - metabolism; chondrocytes; microRNA-140; osteoarthritis; synovial fluid
• ISSN: 1001-9294
• DOI: 10.1016/S1001-9294(17)30002-0

Objective To investigate the expression of miRNA-140 in chondrocytes and synovial fluid ofosteoarthritis (OA) patients, and explore the relationship between the miRNA-140 expression and OAseverity.Methods This study enrolled 30 OA patients who underwent total knee arthroplasty for chondrocytessampling and 30 OA patients who underwent intra-articular injection for synovial fluid sampling. All OApatients were grouped into mild [Kellgren and Lawrence (KL) grade 1-2], moderate (KL grade 3) and severe(KL grade 4), with 10 in each subgroups for each sampling purposes. 7 non-OA patients and 10 patients withknee injury were collected for cartilage and synovial fluid sampling respectively as control groups.Chondrocytes were isolated from the cartilage tissue and cultured in vitro. Quantitative real time PCR formiRNA-140 in chondrocytes and synovial fluid were performed, and the U6 snRNA was used as internalcontrol. The expression difference of miRNA-140 among groups and correlation between the expression andthe KL grade of OA were analysed using one-way ANOVA and Spearman test respectively.