The preventive effects of native probiotic and postbiotic on inflammation and oxidative stress in DSS-induced colitis with normal diet: Which of these agents may offer greater advantages?

• Published in: Heliyon Vol. 10; no. 17; p. e37279
• Main Authors: Rezaie, Niloofar, Aghamohammad, Shadi, Haj Agha Gholizadeh Khiavi, Elham, Khatami, Shohreh, Sohrabi, Aria, Rohani, Mahdi
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 15.09.2024; Elsevier
• Subjects: Inflammatory status; Normal diet; Postbiotic; Probiotic; ROS imbalance; Probiotic; Inflammatory status; Normal diet; Postbiotic; ROS imbalance
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2024.e37279

Maintaining a well-rounded and healthy diet is essential to promote the well-being and optimal performance of the body, especially for those suffering from Inflammatory Bowel Disease (IBD). The objective of this study is to examine whether probiotics and postbiotics can modulate oxidative stress and inflammation, and to evaluate the properties of these compounds. A total of eighty eight strains of Lactobacillus and Bifidobacterium were assessed for their antioxidant activities. C57BL/6 mice were allocated into four groups: normal diet (ND) + PBS, ND + DSS, ND + DSS + 10⁹ cfu/ml of probiotics, and ND + DSS + 10⁹ cfu/ml of postbiotics. Biochemical antioxidant assays, along with colitis indices, were evaluated. The ELISA assay was conducted to measure oxidant/antioxidant properties and cytokines. Additionally, the genes enrolled in NF-kB and Nrf2 signaling pathways was analyzed. In comparison to the groups exposed to DSS alone, mice that received our native agents in addition to DSS demonstrated an improvement in the negative effects induced by DSS on DAI and pathological scores, as well as on colon length and body weight. The levels of cytokines and antioxidant markers have also been normalized following the administration of our native agents, along with molecular markers. It should also be noted that our native postbiotic was able to develop more pronounced and significant anti-inflammatory and antioxidant effects in comparison to the probiotic strains. In this study, our native postbiotic has demonstrated a more pronounced ability to exhibit antioxidant and anti-inflammatory effects. This finding is particularly important for individuals with impaired immune function, for whom the use of live bacteria could be risky. Therefore, the utilization of agents like probiotics and postbiotics, which come with minimal side effects in compared to chemical drugs, could be essential in managing symptoms in IBD patients. [Display omitted] •Native postbiotics excel probiotics in oxidative stress and inflammatory modulation.•Probiotics and postbiotics show anti-inflammatory properties in DSS-induced colitis.•Postbiotics offer a safer option for managing inflammation in IBD patients.•Probiotics and postbiotics are promising for addressing oxidative stress in IBD.