Evaluation of the immune response to CRA and FRA recombinant antigens of Trypanosoma cruzi in C57BL/6 mice

• Published in: Revista da Sociedade Brasileira de Medicina Tropical Vol. 36; no. 4; pp. 435 - 440
• Main Authors: Pereira, Valéria Rêgo Alves, de Lorena, Virginia Maria Barros, Nakazawa, Mineo, da Silva, Ana Paula Galvão, Montarroyos, Ulisses, Correa-Oliveira, Rodrigo, Gomes, Yara de Miranda
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira de Medicina Tropical 01.07.2003; Sociedade Brasileira de Medicina Tropical - SBMT
• Subjects: Animals; Antigens - administration & dosage; Antigens - immunology; Antigens, Protozoan - administration & dosage; Antigens, Protozoan - immunology; Hypersensitivity; Hypersensitivity, Immediate - immunology; Immune response; Immunity, Cellular - immunology; Immunization; Immunoglobulin G - analysis; Male; Mice; Mice, Inbred C57BL; TROPICAL MEDICINE; Trypanosoma cruzi - immunology; Linfoproliferação; Immunization; Isotypes; Recombinant antigens; Antígenos recombinantes; Lymphoproliferation; Isotipos; Trypanosoma cruzi; Imunização
• ISSN: 0037-8682; 1678-9849; 0037-8682; 1678-9849
• DOI: 10.1590/s0037-86822003000400001

Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Significant differences were observed between incorporation of [ H]- thymidine by spleen cell stimulated in vitro with CRA or FRA and the control group. These results suggest that CRA and FRA could be involved in mechanisms of resistance to Trypanosoma cruzi infection.