Isolation, characterization and antioxidant activity of polysaccharide from Schisandra sphenanthera

• Published in: Carbohydrate polymers Vol. 105; pp. 26 - 33
• Main Authors: Zhao, Ting, Mao, Guanghua, Feng, Weiwei, Mao, Riwen, Gu, Xiaoyun, Li, Ting, Li, Qian, Bao, Yongtuan, Yang, Liuqing, Wu, Xiangyang
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier 25.05.2014
• Subjects: Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - metabolism; Applied sciences; Biological and medical sciences; Dose-Response Relationship, Drug; Exact sciences and technology; Free Radical Scavengers - metabolism; General and cellular metabolism. Vitamins; Medical sciences; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - metabolism; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - metabolism; Schisandra; Starch and polysaccharides; Molecular structure; Chemical isolation; Medicinal plant; Radical trapping; Structural elucidation; Scavenging efficiency; Activity concentration relation; Dicotyledones; Angiospermae; Schisandraceae; DPPH; Antioxidant activity; Methylation analysis; Antioxidant; Experimental study; Water soluble compound; Plant origin; Triple helical structure; Schisandra sphenanthera Rehd. et Wits; Oside polymer; Spermatophyta; Aqueous solution; NMR spectroscopy; Polysaccharide; Conformation
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2014.01.059

A water-soluble polysaccharide (SSPP11) from Schisandra sphenanthera was purified by DEAE-cellulose and Sephadex G-100 columns. Structure of SSPP11 and its antioxidant activity was evaluated. Results showed that SSPP11 has a molecular weight of 5.3×10(3)Da and is composed of Man, Glu and Gal. A linkage analysis and NMR study revealed that SSPP11 has a backbone of →1)-d-Man-(6→, →1)-d-Manp-(2→, →1)-d-Glup(4→, →1)-d-Glup-(6→, →1)-d-Galp-(4→, →1)-d-Galp-(4,6→ and →1)-d-Manp-(3,6→, with Man, Glu and Gal, which are distributed in branched chains. The Congo red absorption test revealed that SSPP11 has a triple helix stereo-configuration. Moreover, antioxidant activity of SSPP11 was stronger than the polysaccharide from Schisandra chinensis (Turcz.) Baill. In sum, this study demonstrates that a moderate molecular weight, triple helix stereo-configuration and higher degree of branching are beneficial for exerting antioxidant activity.