Method for evaluation of ionic interactions in liquid chromatography

• Published in: Journal of Chromatography A Vol. 1625; p. 461301
• Main Authors: Kadlecová, Zuzana, Kalíková, Květa, Folprechtová, Denisa, Tesařová, Eva, Gilar, Martin
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 16.08.2020
• Subjects: Inorganic ions; Ionic interactions; Mixed-mode; Reversed-phase; Silanols; Mixed-mode; Inorganic ions; Silanols; Reversed-phase; Ionic interactions
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2020.461301

•We propose method for evaluation of ionic interactions of liquid chromatography.•Permanently ionized organic and inorganic ions were utilized as test analytes.•Aqueous ammonium dichloroacetate mobile phases are used in range pH 2.5–9.0.•The method is applicable for characterization of mixed mode columns. In this work we utilized basic and acidic analytes to investigate the ionic interaction participation in retention behavior of selected reversed-phase and polar columns. The test analytes included nitrate, benzenesulfonate and trimethylphenylammonium ions. The fully aqueous mobile phase comprising 10 mM dichloroacetic acid buffered with ammonia solution to desirable pH was used for retention experiments. Developed method was utilized to study the ionic interactions of stationary phases in pH range between 2.5 and 9.0. We demonstrate that selected sorbents used for reversed-phase and hydrophilic interaction chromatography separations exhibit cation- or anion-exchange interactions. We compare the results to novel Atlantis PREMIER BEH C18 AX mixed-mode column that combines reversed-phase and anion-exchange interaction modes. We evaluated the relative retention strength of selected columns for anionic and cationic analytes.