Ki- ras and c- myc Oncogene Expression Measured by Coamplification Polymerase Chain Reaction

Ki- ras and c- myc oncogene mRNA in carcinoma tissue was quantitatively detected by the coamplification polymerase chain reaction. After the reverse transcription of the mRNA mixture with random hexanucleotide primer the coamplification polymerase chain reaction of the oncogene and the internal stan...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 201; no. 2; pp. 574 - 580
Main Authors Sugimoto, T., Tsukamoto, F., Fujita, M., Takai, S.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.06.1994
Subjects
Actins - biosynthesis
Base Sequence
Cell Line
DNA Primers
Gastric Mucosa - metabolism
Gene Expression
Genes, myc
Genes, ras
Humans
Kinetics
Molecular Sequence Data
Oligonucleotides, Antisense
Polymerase Chain Reaction - methods
Proto-Oncogenes
RNA, Messenger - biosynthesis
Stomach Neoplasms
Templates, Genetic
Tumor Cells, Cultured
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Summary:Ki- ras and c- myc oncogene mRNA in carcinoma tissue was quantitatively detected by the coamplification polymerase chain reaction. After the reverse transcription of the mRNA mixture with random hexanucleotide primer the coamplification polymerase chain reaction of the oncogene and the internal standard β-actin cDNA was done. The amount of oncogene mRNA was calculated from the coamplified product ratio. Ki- ras expression in a human gastric cancer strain H-111 was estimated to 5 percent of β-actin. The expression of c- myc mRNA in colorectal carcinoma tissue was observed to be ten to one hundred times higher than normal mucosa. Gene expression was compared numerically in mole/liter without using hybridization and radioactive probes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1994.1740