Enzyme Immunometric Assay for L-Thyroxine Using Direct Ultraviolet Irradiation

A new enzyme immunometric assay for L-thyroxine using uv irradiation as a cross-linking procedure is described. L-Thyroxine in plasma samples is immunocaptured by a monoclonal anti-L-thyroxine antibody coated on 96-well microtiter plates. After uv irradiation and methanol treatment, the covalently l...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 225; no. 1; pp. 34 - 38
Main Authors Etienne, E., Creminon, C., Lamourette, P., Grassi, J., Pradelles, P.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 10.02.1995
Subjects
Antibodies, Monoclonal
Cross Reactions
Cross-Linking Reagents
Epitopes
Humans
Immunoenzyme Techniques
Reproducibility of Results
Sensitivity and Specificity
Thyroxine - blood
Ultraviolet Rays
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Summary:A new enzyme immunometric assay for L-thyroxine using uv irradiation as a cross-linking procedure is described. L-Thyroxine in plasma samples is immunocaptured by a monoclonal anti-L-thyroxine antibody coated on 96-well microtiter plates. After uv irradiation and methanol treatment, the covalently linked L-thyroxine is measured using the same monoclonal anti-L-thyroxine antibody labeled with acetylcholinesterase. A minimal detectable concentration of 4.8 nmol/liter was observed with a coefficient of variation less than 16% in the 20-320 nmol/liter. Specificity of the assay was very satisfying and a good correlation ( r = 0.959) was noted for 33 human plasma samples between this assay and a commercial competitive radioimmunoassay.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1995.1104