Bacterial vectors for the delivery of tumor antigens

The use of bacterial vectors, which offer ease of production and efficiency, has become an important mechanism for the delivery of protein antigens to antigen-presenting cells (APCs) in vivo. Proof of concept studies has been carried out utilizing different bacteria in various cancer models with som...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1139; p. 429
Main Authors Wang, Yan, Toussaint, Bertrand, Le Gouëllec, Audrey
Format Journal Article
LanguageEnglish
Published United States 2014
Subjects
Animals
Antigens, Neoplasm - genetics
Antigens, Neoplasm - immunology
Cancer Vaccines - genetics
Cancer Vaccines - immunology
Cell Line
Cloning, Molecular
Genetic Vectors - genetics
Mice
Plasmids - genetics
Pseudomonas aeruginosa - genetics
Transformation, Genetic
Vaccination
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Summary:The use of bacterial vectors, which offer ease of production and efficiency, has become an important mechanism for the delivery of protein antigens to antigen-presenting cells (APCs) in vivo. Proof of concept studies has been carried out utilizing different bacteria in various cancer models with some in clinical trials. Here we described the way to prepare Pseudomonas aeruginosa (P. aeruginosa) vaccines based on a virulence-attenuated strain to test the efficacy of different fragments of a well-known tumor antigen. This protocol could be applied to efficacy studies in murine models of human cancers.
ISSN:1940-6029
DOI:10.1007/978-1-4939-0345-0_33