Osteopontin mRNA in the kidney on an experimental rat model of renal stone formation without renal failure

We had sequenced a cDNA of calcium oxalate urinary stone protein extracted with 0.1 M EDTA. cDNA sequences showed complete homology between urinary stone protein and human osteopontin (OPN, bone sialoprotein I). In the present study, we investigated the expression of OPN mRNA in the rat kidney in an...

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Bibliographic Details
Published inUrologia internationalis Vol. 55; no. 1; p. 6
Main Authors Umekawa, T, Yamate, T, Amasaki, N, Kohri, K, Kurita, T
Format Journal Article
LanguageEnglish
Published Switzerland 1995
Subjects
Acute Kidney Injury - chemically induced
Acute Kidney Injury - complications
Acute Kidney Injury - metabolism
Animals
Blotting, Northern
Immunohistochemistry
In Situ Hybridization
Kidney - metabolism
Kidney Calculi - complications
Kidney Calculi - metabolism
Male
Osteopontin
Phosphoproteins - analysis
Phosphoproteins - genetics
Rats
Rats, Wistar
RNA, Messenger - analysis
Sialoglycoproteins - analysis
Sialoglycoproteins - genetics
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Summary:We had sequenced a cDNA of calcium oxalate urinary stone protein extracted with 0.1 M EDTA. cDNA sequences showed complete homology between urinary stone protein and human osteopontin (OPN, bone sialoprotein I). In the present study, we investigated the expression of OPN mRNA in the rat kidney in an experimental model of renal stone formation using glyoxylic acid and 1,25-dihydroxyvitamin D3 (D3). In the renal stone formation model with and without renal failure, OPN mRNA was shown to be localized by in situ hybridization using an OPN cRNA probe mainly in the distal convoluted tubule of the kidney, and was enhanced compared with the normal control which was sporadically positive. By Northern blot analysis, the expression of OPN mRNA was shown to be increased by about 5.2-fold in the renal stone formation model and 2.3-fold in D3-administered rats compared with controls. However, no change in the expression of OPN mRNA was observed in an acute renal failure model induced by gentamicin or in rats administered glycoxylic acid alone. Therefore, the promotion of OPN mRNA expression was intrinsic to this stone formation model, and not secondary to acute renal failure because of obstruction by microcrystals in the renal tubules or gentamicin.
ISSN:0042-1138
DOI:10.1159/000282737