Immobilization of biomolecules via ruthenium-catalyzed functionalization of the surface of silica with a vinylsilane

A new ruthenium complex catalyzed procedure for the efficient O-silylation of an SiO–H group on the silica surface of glass beads with controlled pores (CPG) using 1-trimethylsiloxy-3-dimethylvinylsilylpropane leading to Si–O–Si bond formation with the evolution of ethylene is described. The formed...

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Bibliographic Details
Published inTetrahedron letters Vol. 54; no. 28; pp. 3605 - 3608
Main Authors Hreczycho, Grzegorz, Chmielewski, Marcin K., Maciejewski, Hieronim, Ratajczak, Tomasz, Marciniec, Bogdan
Format Journal Article
LanguageEnglish
Published OXFORD Elsevier Ltd 10.07.2013
Elsevier
Subjects
Automation
Beads
Biomolecules
Chemistry
Chemistry, Organic
CPG support
Ethylene
Fluorescent probe
Fragments
Hydroxyl groups
Physical Sciences
Ruthenium catalysts
Science & Technology
Silica glass
Silica surface
Silicon dioxide
Vinylsilane
CPG support
Vinylsilane
Ruthenium catalysts
Silica surface
Fluorescent probe
ROOM-TEMPERATURE
ROUTE
O-SILYLATION
ALCOHOL
CARBON-HYDROGEN BONDS
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Summary:A new ruthenium complex catalyzed procedure for the efficient O-silylation of an SiO–H group on the silica surface of glass beads with controlled pores (CPG) using 1-trimethylsiloxy-3-dimethylvinylsilylpropane leading to Si–O–Si bond formation with the evolution of ethylene is described. The formed linker contains alkyl hydroxyl groups which can be reacted with a nucleoside phosphoramidite unit. The CPG support containing alkyl hydroxyl groups is successfully applied in automatic chemical synthesis of DNA fragments.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0040-4039
1873-3581
DOI:10.1016/j.tetlet.2013.04.078