A comparison study on microwave-assisted extraction of Potentilla anserina L. polysaccharides with conventional method: Molecule weight and antioxidant activities evaluation

• Published in: Carbohydrate polymers Vol. 80; no. 1; pp. 84 - 93
• Main Authors: Wang, Junlong, Zhang, Ji, Zhao, Baotang, Wang, Xiaofang, Wu, Yiqian, Yao, Jian
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 25.03.2010; Elsevier
• Subjects: Antioxidant; Applied sciences; Biological and medical sciences; Box–Behnken design; Chain conformation; Exact sciences and technology; General and cellular metabolism. Vitamins; Medical sciences; Microwave-assisted extraction; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; Potentilla anserina polysaccharides; Starch and polysaccharides; Chain conformation; Microwave-assisted extraction; Box–Behnken design; Potentilla anserina polysaccharides; Antioxidant; Rosaceae; Metal ion; Solvent extraction; Optimization; Medicinal plant; Radical trapping; Binding capacity; Scavenging efficiency; Dicotyledones; Angiospermae; Microwave irradiation; Hydroxyl radicals; Chemical composition; Molecular mass; DPPH; Iron II; Experimental study; Biological activity; Operating conditions; Plant origin; Oside polymer; Spermatophyta; Superoxide radicals; Box-Behnken design
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2009.10.073

The conventional extraction methods for polysaccharides were time-consuming, laborious and energy-consuming. Microwave-assisted extraction (MAE) technique was employed for the extraction of Potentilla anserina polysaccharides (PAP MAE), which is a traditional Chinese functional food. The extracting parameters of PAP MAE were optimized by Box–Behnken design. The optimum conditions were ratio of water to raw material 14.5:1, microwave power 369 W, extraction temperature 63.3 °C and extraction time 76.8 min with an enhanced yield of 13.33%. In microwave heating process, a sharp decrease in M w was detected in SEC-LLS measurement. A d f value of 3.03 indicated that PAP MAE exhibited a compact hard sphere conformation in an aqueous solution. Furthermore, it showed stronger antioxidant activities compared with hot water extraction by evaluating in superoxide radical ( O 2 - ), hydroxyl radical ( OH), 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, reducing power and metal chelating assay. The data obtained clearly showed that the molecular weights played a more important role in antioxidant activities.