Medium optimization, preliminary characterization and antioxidant activity in vivo of mycelial polysaccharide from Phellinus baumii Pilát

• Published in: Carbohydrate polymers Vol. 81; no. 3; pp. 533 - 540
• Main Authors: Luo, Jianguang, Liu, Jun, Sun, Yi, Ye, Hong, Zhou, Chunhong, Zeng, Xiaoxiong
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 07.07.2010; Elsevier
• Subjects: Antioxidant activity in vivo; Applied sciences; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General and cellular metabolism. Vitamins; Medical sciences; Medium components; Methods. Procedures. Technologies; Mycelial polysaccharide; Natural polymers; Pharmacology. Drug treatments; Phellinus baumii Pilát; Physicochemistry of polymers; Starch and polysaccharides; Statistical experimental design; Medium components; Antioxidant activity in vivo; Mycelial polysaccharide; Statistical experimental design; Phellinus baumii Pilát; Molecular structure; Biosynthesis; Response surface methodology; Optimization; Basidiomycota; Medicinal plant; Fungi; Chinese medicine; Enzymatic activity; Folk medicine; Culture medium; Rodentia; Antioxidant; Experimental study; Biological activity; Operating conditions; In vivo; Vertebrata; Mammalia; Submerged culture; Mouse; Experimental design; Animal; Plant origin; Microorganism culture; Oside polymer; Factorial design
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2010.03.010

Optimization of medium ingredients for the production of mycelial polysaccharides from Phellinus baumii Pilát (PBMP) in submerged culture, preliminary characterization and the evaluation of antioxidant activity in vivo of PBMP were carried out. An optimal medium for PBMP production was obtained through a 2 (7−3) fractional factorial design and a central composite design in response surface methodology as follows (g/l): glucose 35.36, yeast extract 2.88, peptone 2.73, MgSO 4 1.47, KH 2PO 4 1, VB 1 0.0075 and diammonium oxalate monohydrate 0.3. The resulting PBMP was composed of d-glucose, d-galactose, l-fucose, d-mannose, and l-rhamnose in a molar ratio of 12.74:1.39:1.00:1.92:0.22. It was demonstrated that the administration of PBMP could increase the activities of antioxidant enzymes, decrease the levels of malondialodehyde, and enhance total antioxidant capabilities in livers and serums of d-galactose-induced mice. The results suggested that PBMP had potent antioxidant activity and could be explored as novel natural antioxidant.