Generation of an S100B homozygous knockout pluripotent stem cell line (WAe009-A-94) by the CRISPR/Cas9 system

S100 calcium binding protein beta (S100B) is an S-100 low molecular weight binding protein that regulates intracellular processes. This protein is involved in myocardial contractility and calcium handling capacity. In this study, a human embryonic stem cell (hESC) line with homozygous S100B knockout...

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Published inStem cell research Vol. 64; p. 102924
Main Authors zhang, Pengxia, Wang, Hongyue, Li, Shengzhen, Guo, Tianwei, Zhang, Rui, Zhou, Ping, Ding, Shaoli
Format Journal Article
LanguageEnglish
Published Elsevier 01.10.2022
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Summary:S100 calcium binding protein beta (S100B) is an S-100 low molecular weight binding protein that regulates intracellular processes. This protein is involved in myocardial contractility and calcium handling capacity. In this study, a human embryonic stem cell (hESC) line with homozygous S100B knockout (S100B-KO) was generated using the CRISPR/Cas9 editing system. This S100B-KO hESC line maintained normal cell morphology and karyotype, expressed pluripotency markers, and could differentiate into cells of all three germ layers.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.102924