Plant regeneration through somatic embryogenesis in the forage grass Caucasian bluestem (Bothriochloa caucasica)

Plantlets were regenerated from cultured seed explants of the forage grass Caucasian bluestem [Bothriochloa caucasica (Trin.) C.E. Hubbard] via somatic embryogenesis. Embryogenic callus was produced in four weeks when surface sterilized seeds were cultured on a medium containing MS-salts, B-5 vitami...

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Bibliographic Details
Published inPlant cell reports Vol. 9; no. 8; pp. 443 - 446
Main Authors Franklin, C.I, Trieu, T.N, Gonzales, R.A
Format Journal Article
LanguageEnglish
Published Germany 01.12.1990
Subjects
Bothriochloa bladhii
callus
carbenicillin
culture media
growth rate
kanamycin
regeneration
somatic embryogenesis
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Summary:Plantlets were regenerated from cultured seed explants of the forage grass Caucasian bluestem [Bothriochloa caucasica (Trin.) C.E. Hubbard] via somatic embryogenesis. Embryogenic callus was produced in four weeks when surface sterilized seeds were cultured on a medium containing MS-salts, B-5 vitamins, 12 mM L-proline, 2% sucrose, 0.8% agar and 5μM 2,4-D. Plantlets were regenerated in 6-8 weeks after culture initiation. Healthy root and shoot systems were produced within three weeks after the plantlets were transferred to a medium lacking 2,4-D. Approximately 95% of the plantlets survived greenhouse acclimation and produced healthy plants and viable seeds. Caucasian bluestem callus cultures exhibit natural resistance to kanamycin. High levels of kanamycin (up to 800 mg/l) did not completely inhibit callus growth. However, the regeneration of healthy-plantlets was completely inhibited by kanamycin even at low levels (50 mg/l).
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00232269