Effect of protein hydrolysates from sardinelle (Sardinella aurita) on the oxidative status and blood lipid profile of cholesterol-fed rats

• Published in: Food research international Vol. 45; no. 1; pp. 60 - 68
• Main Authors: Ben Khaled, Hayet, Ghlissi, Zohra, Chtourou, Yassine, Hakim, Ahmed, Ktari, Naourez, Fatma, Makni Ayadi, Barkia, Ahmed, Sahnoun, Zouheir, Nasri, Moncef
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.01.2012; Elsevier
• Subjects: Biological and medical sciences; Cholesterol-enriched diet; Feeding. Feeding behavior; Food industries; Fundamental and applied biological sciences. Psychology; Hypocholesterolemic; Protein hydrolysates; Sardinelle; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Sardinelle; Hypocholesterolemic; Cholesterol-enriched diet; Protein hydrolysates; Rat; Rodentia; Lipids; Sardinella aurita; Cholesterol; Profile; Protein; Blood; Vertebrata; Mammalia; Hydrolysate; Diet; Pisces
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2011.10.003

This study was designed to test the hypolipidemic properties and antioxidative activities of sardinelle (Sardinella aurita) protein hydrolysates (SPHs) obtained by treatment with crude enzyme preparations from Bacillus pumilus A1(SPHA1), Bacillus mojavensis A21(SPHA21) and crude enzyme extract from sardinelle viscera (SPHEE). Wistar rats were fed during 7weeks a standard laboratory diet, a cholesterol-enriched diet (1%) or a cholesterol SPH-enriched diet. The hypercholesterolemic diet induced the elevation of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C). Supplementing cholesterol-enriched diet with SPHs or whole sardinelle protein (WSP) at a concentration of 5% (w/w) increased the serum level of high-density lipoprotein cholesterol (HDL-C) and HDL-C/TC ratio and decreased the serum levels of TC, TG, LDL-C and LDL-C/HDL-C ratio significantly. The thiobarbituric acid-reactive substances (TBARS) level, as an indicator of lipid peroxidation, and the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were examined. The hepatic antioxidant enzymes activities were significantly decreased and the malondialdehyde (MDA) level was increased in rats fed a cholesterol-enriched diet compared to those fed a standard diet. The treatment of hypercholesterolemic (HCD) diet rats with SPHs reduced the MDA concentration and increased the antioxidant enzyme activities. These results suggested that the hypolipidemic effect of SPHs might be due to their abilities to lower serum TC, TG, and LDL-C levels as well as to their antioxidant activities preventing the lipid peroxidation process.