Assessing substrate acceptance and enantioselectivity of yeast reductases in reactions with substituted α-keto β-lactams

A number of yeast ( Saccharomyces cerevisiae) strains and yeast reductases overexpressed in Escherichia coli were investigated as bioreductants for several α-keto-β-lactams substituted with aryl and alkyl groups in the 4-position. Two of the yeast reductases were found to accept a majority of the su...

Full description

Saved in:
Bibliographic Details
Published inJournal of molecular catalysis. B, Enzymatic Vol. 32; no. 5; pp. 167 - 174
Main Authors Yang, Yan, Kayser, Margaret M., Rochon, Fernande D., Rodríguez, Sonia, Stewart, Jon D.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.03.2005
Elsevier Science
Subjects
Bioconversions. Hemisynthesis
Biological and medical sciences
Bioreductions
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Yeast reductases
Yeast-catalyzed reductions
α-Hydroxy β-lactams
α-Keto β-lactams
α-Keto β-lactams
Yeast-catalyzed reductions
Yeast reductases
Bioreductions
α-Hydroxy β-lactams
Yeast
Enantioselectivity
Enzyme
β-Lactams
Fungi
Chemical reduction
Biotransformation
Ascomycetes
Oxidoreductases
Saccharomyces cerevisiae
Thallophyta
Reductase
Online AccessGet full text

Cover

More Information
Summary:A number of yeast ( Saccharomyces cerevisiae) strains and yeast reductases overexpressed in Escherichia coli were investigated as bioreductants for several α-keto-β-lactams substituted with aryl and alkyl groups in the 4-position. Two of the yeast reductases were found to accept a majority of the substrates tested, while others were more limited. Although none of the reductases investigated showed both diastero- and enantio-selectivity under the screening conditions, these bioreagents are still useful in reductions of α-keto β-lactams.
ISSN:1381-1177
1873-3158
DOI:10.1016/j.molcatb.2004.10.005